5ZBR
Crystal Structure of Kinesin-3 KIF13B motor domain in AMPPNP form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-12 |
Detector | ADSC QUANTUM 1 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 32 |
Unit cell lengths | 110.303, 110.303, 95.124 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.105 - 2.000 |
R-factor | 0.1902 |
Rwork | 0.189 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbj |
RMSD bond length | 0.007 |
RMSD bond angle | 0.935 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.072 | 0.815 |
Number of reflections | 85440 | 8768 |
<I/σ(I)> | 23.8 | 3.2 |
Completeness [%] | 97.7 | 100 |
Redundancy | 8 | |
CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.1 | 298 | 0.2M sodium citrate dihydrate, 0.1M Bis-Tris propone, 17% w/v PEG 3350 |