5Z6W

Crystal structure of paFAN1 bound to 2nt 5'flap DNA with gap with Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	80.874, 104.969, 105.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 3.200
R-factor	0.185
Rwork	0.182
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r8a
RMSD bond length	0.013
RMSD bond angle	1.155
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1-2155_1069: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.981	0.981
Rpim	0.042	0.414
Number of reflections	15512	762
<I/σ(I)>	16.19
Completeness [%]	99.9
Redundancy	6.4
CC(1/2)	0.998	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	291	0.1M HEPES(pH7.2), 25%(w/v) PEG 8000, 30mM Gly-gly-gly