5Z5E
Crystal structure of the Glycyl-tRNA synthetase (GlyRS) in Nanoarchaeum equitans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2012-10-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.410, 121.096, 133.635 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 48.198 - 2.098 |
| R-factor | 0.2029 |
| Rwork | 0.201 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ati |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.839 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.098 | 2.098 |
| Rmerge | 0.070 | 0.323 |
| Number of reflections | 70618 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 95.9 | 88.4 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277.15 | 0.05M sodium acetate trihydrate (pH 4.6), 1M ammonium sulfate and 3mg/ml protein |
