5Z5A
Crystal structure of Tk-PTP in the active form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2017-06-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.987 |
Spacegroup name | P 61 |
Unit cell lengths | 102.722, 102.722, 83.805 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.960 - 1.800 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.20900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z59 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.964 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.799 | 1.800 |
Number of reflections | 45504 | |
<I/σ(I)> | 31.6 | |
Completeness [%] | 97.6 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.03M citric acid, 0.07M Bis-Tris propane (pH 7.6), 8%(w/v) polyethylene glycol 3350, 0.08M spermine tetrahydrochloride |