5Z4P

Crystal structure of tubulin-stathmin-TTL-Compound TCA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	103.832, 154.843, 181.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.636 - 2.500
R-factor	0.2223
Rwork	0.222
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o2b
RMSD bond length	0.009
RMSD bond angle	1.133
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.640	2.665
High resolution limit [Å]	2.500	2.500
Number of reflections	101936
<I/σ(I)>	9.88
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	6% PEG 4000, 8% glycerol, 100mM MES, 30mM CaCl2, 30mM MgCl2, pH 6.7