5Z3M

Crystal structure of Low Molecular Weight Phosphotyrosine phosphatase (VcLMWPTP-2) from Vibrio choleraeO395

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	125.912, 51.867, 83.896
Unit cell angles	90.00, 103.62, 90.00

Refinement procedure

Resolution	19.402 - 2.600
R-factor	0.2538
Rwork	0.252
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lrq
RMSD bond length	0.005
RMSD bond angle	1.061
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.402	2.692
High resolution limit [Å]	2.600	2.600
Number of reflections	31285
<I/σ(I)>	8
Completeness [%]	98.9
Redundancy	2.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	AMS, HEPES, GLYCEROL, MOPS, NaCl