5Z2T

Crystal structure of DNA-bound DUX4-HD2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-15
Detector	MAR CCD 130 mm
Wavelength(s)	0.9747
Spacegroup name	P 41 21 2
Unit cell lengths	51.610, 51.610, 166.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.297 - 2.623
R-factor	0.2779
Rwork	0.276
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z2s
RMSD bond length	0.005
RMSD bond angle	0.826
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.300	2.760
High resolution limit [Å]	2.620	2.620
Number of reflections	7363
<I/σ(I)>	11.5
Completeness [%]	99.9
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	PEG 8000