5YY9

Crystal structure of Tandem Tudor Domain of human UHRF1 in complex with LIG1-K126me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	27.105, 97.349, 132.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.228 - 2.653
R-factor	0.2367
Rwork	0.231
R-free	0.28770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3db3
RMSD bond length	0.004
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.120	0.700
Number of reflections	40019
<I/σ(I)>	8.5	2.4
Completeness [%]	97.0	95.4
Redundancy	3.8	3.8
CC(1/2)	0.990	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	00 mM Tris-HCl (pH 7.0), 200 mM tri-potassium phosphate and 20% (w/v) PEG 3350