5YVT
Crystal structure of the alpha gamma heterodimer of human IDH3 in complex with Mg(2+) and NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 118.027, 118.027, 142.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.23637 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5grh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.296 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 45428 | |
| <I/σ(I)> | 22.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES-Na, pH 7.5, 50 mM MgCl2, 30% (v/v) PEGMME 550 |
