5YST

RanM189D in complex with RanBP1-CRM1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97921
Spacegroup name	P 43 21 2
Unit cell lengths	104.884, 104.884, 307.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.040
R-factor	0.2106
Rwork	0.209
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hat
RMSD bond length	0.006
RMSD bond angle	1.114
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.040	5.540	2.040
Rmerge	0.156	0.102
Rmeas	0.163	0.107
Rpim	0.058	0.031	0.766
Number of reflections	110107	5999	5417
<I/σ(I)>	3
Completeness [%]	100.0	100	100
Redundancy	12.9	12.4	13.1
CC(1/2)		0.994	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	18% PEG 3350, 200mM Ammonium Nitrate, 100mM Bis-Tris pH 6.6