5YS3
1.8 angstrom crystal structure of Succinate-Acetate Permease from Citrobacter koseri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 |
| Unit cell lengths | 79.456, 79.456, 89.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.728 - 1.823 |
| R-factor | 0.1619 |
| Rwork | 0.160 |
| R-free | 0.19850 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.129 |
| Data reduction software | HKL-2000 (v1.0) |
| Data scaling software | HKL-2000 (v1.0) |
| Phasing software | SHELXD (v1.0) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.800 | |
| Number of reflections | 51162 | |
| <I/σ(I)> | 17.5 | 1.9 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 298 | PEG 400, CaAc2, NaCl |
