5YQ2
Crystal structure of E.coli aminopeptidase N in complex with Puromycin aminonucleoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-10-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.223, 120.223, 170.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.1579 |
| Rwork | 0.157 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hpo |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.509 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK (2.2.0) |
| Phasing software | MOLREP (11.4.05; 02.04.2016) |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.044 | 0.030 | 0.324 |
| Rmeas | 0.049 | 0.034 | 0.358 |
| Rpim | 0.021 | 0.015 | 0.152 |
| Total number of observations | 1040498 | ||
| Number of reflections | 186872 | 19224 | 18535 |
| <I/σ(I)> | 19.6 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 5.6 | 5 | 5.4 |
| CC(1/2) | 0.998 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0M Sodium malonate |
