5YPD
Mycobacterium Tuberculosis Methionine aminopeptidase type 1c (C105N mutant) in complex with Methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-09-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.226, 44.909, 57.295 |
Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
Resolution | 28.570 - 1.620 |
R-factor | 0.1461 |
Rwork | 0.144 |
R-free | 0.18180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yj3 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.106 |
Data reduction software | DENZO (2.3.1) |
Data scaling software | SCALEPACK (2.2.0) |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.570 | 30.000 | 1.680 |
High resolution limit [Å] | 1.620 | 3.490 | 1.620 |
Rmerge | 0.044 | 0.037 | 0.200 |
Rmeas | 0.062 | 0.052 | 0.283 |
Rpim | 0.044 | 0.037 | 0.200 |
Number of reflections | 29506 | 5852 | 5139 |
<I/σ(I)> | 34 | ||
Completeness [%] | 90.7 | 94.7 | 83.2 |
Redundancy | 1.9 | 1.9 | 1.9 |
CC(1/2) | 0.996 | 0.993 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M Hepes, 27% Peg 3350, 3% Glycerol |