5YJJ

Crystal structure of PNPase from Staphylococcus epidermidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	93.190, 93.160, 143.150
Unit cell angles	73.54, 87.61, 60.07

Refinement procedure

Resolution	73.040 - 2.200
R-factor	0.3
Rwork	0.237
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cdi
RMSD bond length	0.006
RMSD bond angle	0.855
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	75.030
High resolution limit [Å]	2.200
Number of reflections	196762
<I/σ(I)>	8.5
Completeness [%]	97.6
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.3	295	0.2M Potassium formate, 20% PEG 3350, pH 7.3