5YJH

Structural insights into periostin functions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 61 2 2
Unit cell lengths	105.529, 105.529, 330.342
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.041 - 2.957
R-factor	0.2148
Rwork	0.213
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ltb
RMSD bond length	0.004
RMSD bond angle	0.610
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.010
High resolution limit [Å]	2.957	2.957
Rmerge	0.114	0.547
Rmeas	0.131	0.626
Rpim	0.062	0.295
Number of reflections	22983	1117
<I/σ(I)>	14.1	2.8
Completeness [%]	96.4	96.5
Redundancy	3.9	4
CC(1/2)		0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium Sulfate. Sodium cacodylate