5YJG

Structural insights into periostin functions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 61 2 2
Unit cell lengths	105.605, 105.605, 331.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.143 - 2.399
R-factor	0.2133
Rwork	0.211
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ltb
RMSD bond length	0.002
RMSD bond angle	0.538
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.399	2.399
Rmerge	0.108	0.689
Rmeas	0.111	0.714
Rpim	0.026	0.179
Number of reflections	43825	2039
<I/σ(I)>	45.3	3
Completeness [%]	99.7	95.8
Redundancy	18.5	14.2
CC(1/2)		0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium Sulfate, MES