5YJ2
Crystal structure of Bacillus sp. TB-90 urate oxidase without dehydration
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 133.073, 143.895, 70.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.114 - 1.710 |
R-factor | 0.1653 |
Rwork | 0.163 |
R-free | 0.19020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.754 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.102 | 0.760 |
Rmeas | 0.107 | |
Rpim | 0.042 | 0.397 |
Number of reflections | 147456 | 7291 |
<I/σ(I)> | 15.8 | 2.38 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.7 | 6.3 |
CC(1/2) | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 16% PEG 8000, 0.1 M Tris-HCl, 0.08 M K2SO4, 2 mM 8-azaxanthine |