5YH3

The structure of hFam20C and hFam20A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	78.469, 192.747, 226.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.557 - 3.300
R-factor	0.2343
Rwork	0.233
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.719
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	75772
<I/σ(I)>	10
Completeness [%]	99.7
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1M BICINE pH 9.0, 2%(v/v) 1,4-Dioxane, 7%(w/v) Polyethylene glycol 20,000