5YH2

The structure of DrFam20C1 and hFam20A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 41 21 2
Unit cell lengths	110.059, 110.059, 461.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.943 - 3.550
R-factor	0.248
Rwork	0.245
R-free	0.29840
RMSD bond length	0.005
RMSD bond angle	0.739
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.550
Number of reflections	35615
<I/σ(I)>	11
Completeness [%]	100.0
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	290	11% v/v 2-Propanol, 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 8% w/v Polyethylene glycol 10,000