5YEW
Structural basis for GTP hydrolysis and conformational change of mitofusin 1 in mediating mitochondrial fusion
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 207.942, 207.942, 107.893 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.22429 |
| Rwork | 0.223 |
| R-free | 0.25733 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 44371 |
| <I/σ(I)> | 14.33 |
| Completeness [%] | 100.0 |
| Redundancy | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 160mM potassium thiocyanate, 16% PEG3350, 1% tacsimate (pH 7.0), 20mM HEPES, 2% PEG5000, 4% 1-propanol |
