5YEC
Crystal structure of Atg7CTD-Atg8-MgATP complex in form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-01-24 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 138.995, 138.995, 134.368 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.142 - 2.147 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.679 |
| Data reduction software | iMOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.420 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.111 | 0.662 |
| Number of reflections | 69987 | |
| <I/σ(I)> | 10 | 1.3 |
| Completeness [%] | 97.1 | 82.9 |
| Redundancy | 10.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 10% polyethylene glycol monoethyl ether 5000, 0.1M HEPES, 5% tacsimate |
