5YD8

Crystal structure of human PCNA in complex with APIM of human ZRANB3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-22
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	83.678, 83.678, 194.928
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.22088
Rwork	0.219
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zvm
RMSD bond length	0.011
RMSD bond angle	1.508
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.380
High resolution limit [Å]	2.300
Rmerge	0.063
Rmeas	0.078
Number of reflections	40892
<I/σ(I)>	15.5
Completeness [%]	99.0
Redundancy	4.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350