5YC5

Crystal structure of human IgG-Fc in complex with aglycan and optimized Fc gamma receptor IIIa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	72.790, 101.420, 122.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.900 - 2.710
R-factor	0.21197
Rwork	0.209
R-free	0.27294
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ay4
RMSD bond length	0.016
RMSD bond angle	1.984
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	2.840
High resolution limit [Å]	2.710	2.710
Rmerge	0.121	0.848
Rpim	0.058	0.370
Number of reflections	25395	3324
<I/σ(I)>	9.6	2
Completeness [%]	100.0	100
Redundancy	5.9	6.2
CC(1/2)	0.995	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293.15	20mM Tris-HCl, 100mM NaCl, 14% PEG 3350