5YC4

Crystal structure of AL3 PHD finger bound to H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 41 2 2
Unit cell lengths	30.775, 30.775, 157.333
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.775 - 2.697
R-factor	0.2304
Rwork	0.227
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fsa
RMSD bond length	0.002
RMSD bond angle	0.562
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.690	7.320	2.700
Rmerge	0.217	0.091	1.035
Rmeas	0.223	0.095	1.062
Rpim	0.050	0.024	0.233
Number of reflections	2491	168	121
<I/σ(I)>	4.9
Completeness [%]	100.0	99.4	100
Redundancy	19.2	14.1	20.5
CC(1/2)		0.998	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1.8M Ammonium citrate tribasic, pH 7.0.