5Y90
MAP2K7 mutant -C218S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-19 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.396, 70.607, 92.155 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.050 - 1.300 |
R-factor | 0.12887 |
Rwork | 0.127 |
R-free | 0.16714 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b2l |
RMSD bond length | 0.023 |
RMSD bond angle | 2.141 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.050 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.028 | 0.878 |
Number of reflections | 84643 | |
<I/σ(I)> | 60.4 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | PEG3350, sodium citrate |