5Y7A
Crystal structure of Pseudomonas fluorescens Kynurenine 3-monooxygenase in complex with L-KYN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.480, 46.513, 71.311 |
| Unit cell angles | 90.00, 104.34, 90.00 |
Refinement procedure
| Resolution | 38.584 - 1.846 |
| R-factor | 0.1759 |
| Rwork | 0.174 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y77 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.335 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.846 |
| Number of reflections | 36572 |
| <I/σ(I)> | 33.2 |
| Completeness [%] | 97.6 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 293 | 0.1 M Bis-Tris at pH 6.5, 18% w/v PEG3350, and 0.2 M NaCl |
