5Y6L

A subcomplex crystal structure of human cytosolic aspartyl-tRNA synthetase and heterotetrameric glutathione transferase-homology domains in multi-tRNA synthetase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	84.535, 98.413, 125.919
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.873 - 2.900
R-factor	0.1828
Rwork	0.176
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bvy 5bmu 5a34
RMSD bond length	0.010
RMSD bond angle	1.075
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	24317	2376
<I/σ(I)>	17.1	2.3
Completeness [%]	99.5	99.6
Redundancy	6.8	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% PEG 3000 Na/K phosphate pH 6.2