5Y6K
Human serum trnasferrin bound to a fluorescent probe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.93001 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 138.012, 155.752, 107.554 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 103.290 - 2.860 |
R-factor | 0.1576 |
Rwork | 0.154 |
R-free | 0.23520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x1b |
RMSD bond length | 0.013 |
RMSD bond angle | 1.381 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 103.290 | 103.290 | 2.950 |
High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
Rmerge | 0.078 | 0.029 | 0.794 |
Rmeas | 0.084 | 0.031 | 0.848 |
Rpim | 0.029 | 0.011 | 0.296 |
Number of reflections | 27148 | 2801 | 2696 |
<I/σ(I)> | 9.8 | ||
Completeness [%] | 99.7 | 97.6 | 100 |
Redundancy | 8 | 7.6 | 8.1 |
CC(1/2) | 0.996 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 298 | PIPES-Na 0.1M pH 6.6, disodium malonate 8mM, PEG 3350 17%, glycerol 18% |