5Y6K

Human serum trnasferrin bound to a fluorescent probe

Summary for 5Y6K

• Entry DOI: 10.2210/pdb5y6k/pdb
• Descriptor: Serotransferrin, MALONATE ION, FE (III) ION, ... (5 entities in total)
• Functional Keywords: fluorescent, probe, transferrin, metal transport
• Biological source: Homo sapiens (Human)
• Cellular location: Secreted: P02787
• Total number of polymer chains: 1
• Total formula weight: 76519.16

Authors

Jiang, N.,Cheng, T.,Wang, M.,Chan, G.C.F.,Jin, L.,Li, H.,Sun, H. (deposition date: 2017-08-12, release date: 2018-01-24, Last modification date: 2024-11-13)

Primary citation

Jiang, N.,Cheng, T.,Wang, M.,Chan, G.C.,Jin, L.,Li, H.,Sun, H.
Tracking iron-associated proteomes in pathogens by a fluorescence approach.
Metallomics, 10:77-82, 2018

PubMed Abstract: Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.

PubMed: 29323384
DOI: 10.1039/c7mt00275k

Experimental method

X-RAY DIFFRACTION (2.86 Å)