5Y6J
Structure of Tomato spotted wilt virus nucleocapsid protein with alternative oligomerization state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.364, 76.514, 98.440 |
| Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
| Resolution | 49.492 - 2.811 |
| R-factor | 0.2338 |
| Rwork | 0.232 |
| R-free | 0.27520 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.471 | |
| Number of reflections | 20050 | |
| <I/σ(I)> | 21.1 | 4.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.2 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.05 M BIS-TRIS pH 7.0; 22% (v/v) Pentaerythritol ethoxylate (15/4 EO/OH); 20% (v/v) glycerol |
