5Y58
Crystal structure of Ku70/80 and TLC1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 |
Unit cell lengths | 116.296, 115.159, 115.847 |
Unit cell angles | 77.43, 78.48, 63.73 |
Refinement procedure
Resolution | 44.107 - 2.800 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.25770 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.587 |
Data scaling software | HKL-3000 |
Phasing software | XDS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.076 | 0.039 | 0.556 |
Rmeas | 0.089 | 0.046 | 0.645 |
Rpim | 0.045 | 0.023 | 0.326 |
Total number of observations | 463727 | ||
Number of reflections | 121941 | 12863 | 12530 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 93.9 | 99.1 | 96 |
Redundancy | 3.8 | 3.9 | 3.8 |
CC(1/2) | 0.996 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole, 10% PEG8000 |
1 | EVAPORATION | 8 | 277 | 0.2 M calcium acetate, 0.1 M imidazole, 10% PEG8000 |