5Y3T
Crystal structure of hetero-trimeric core of LUBAC: HOIP double-UBA complexed with HOIL-1L UBL and SHARPIN UBL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-03-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.902, 59.732, 58.554 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 48.190 - 2.400 |
| R-factor | 0.18971 |
| Rwork | 0.186 |
| R-free | 0.23961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dbg |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.703 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.092 | 0.520 |
| Number of reflections | 19275 | |
| <I/σ(I)> | 14.2 | 1.9 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Magnesium sulfate, MES |
