5Y37
Crystal structure of GBS GAPDH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 190 |
Detector technology | CCD |
Collection date | 2014-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 144.300, 156.740, 67.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.983 - 1.360 |
R-factor | 0.1665 |
Rwork | 0.166 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qx6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_2481) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Number of reflections | 326358 | 50753 |
<I/σ(I)> | 15.87 | |
Completeness [%] | 99.3 | 96.2 |
Redundancy | 6.83 | 6.1 |
CC(1/2) | 0.999 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 2M Ammonium Sulfate, 0.1 M Tris-HCl, pH 8.5 |