Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-31 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.975, 81.175, 91.480 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 45.729 - 2.701 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd6 4dep 3o4o |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.262 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.429 | |
| Number of reflections | 32256 | |
| <I/σ(I)> | 12.1 | 3.2 |
| Completeness [%] | 98.7 | 95.9 |
| Redundancy | 5.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15% PEG4 000, 0.1 M MES, PH 6.0 |
