5Y20

Crystal structure of AL1 PHD finger bound to H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	46.168, 46.168, 70.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.568 - 2.409
R-factor	0.1685
Rwork	0.161
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fsa
RMSD bond length	0.007
RMSD bond angle	1.002
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine: 1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.221	0.087	0.844
Rmeas	0.225	0.089	0.859
Rpim	0.043	0.018	0.162
Number of reflections	3265
<I/σ(I)>	3.8
Completeness [%]	100.0	100	100
Redundancy	26.5	21.4	27.7
CC(1/2)		0.999	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.1M Tris pH 8., 3.0M NaCl