5Y1Z

Crystal structure of ZMYND8 PHD-BROMO-PWWP tandem in complex with Drebrin ADF-H domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9780
Spacegroup name	P 21 21 21
Unit cell lengths	54.183, 138.095, 161.164
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.988 - 2.676
R-factor	0.1808
Rwork	0.178
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cos 1x67
RMSD bond length	0.009
RMSD bond angle	0.941
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.092	0.036	0.951
Rmeas	0.100	0.039	1.031
Rpim	0.038	0.015	0.392
Number of reflections	34299		1638
<I/σ(I)>	11.1
Completeness [%]	98.9	98.8	94.5
Redundancy	6.5	6.6	6.3
CC(1/2)		0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 6.0 and 23% w/v polyethylene glycol 3,350