5Y1I
The crystal structure of GfsF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.080, 102.870, 114.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.490 - 2.000 |
R-factor | 0.19601 |
Rwork | 0.194 |
R-free | 0.23595 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z36 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.819 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.190 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.109 | 0.578 |
Number of reflections | 50746 | 6781 |
<I/σ(I)> | 8 | 2.2 |
Completeness [%] | 94.2 | 87.7 |
Redundancy | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 299 | 0.2 M KCl, 0.1 M Tris-HCl (pH 8.5) and 27.5% PEG3350 |