5Y16

Crystal structure of human DUSP28(Y102H)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2017-07-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	79.341, 79.341, 90.549
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.634 - 2.399
R-factor	0.1923
Rwork	0.187
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5y15
RMSD bond length	0.008
RMSD bond angle	1.033
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.309
Number of reflections	12461
<I/σ(I)>	22.2
Completeness [%]	93.5	89.4
Redundancy	3.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	100 mM Tris-HCl pH 8.5, 100 mM NaCl, 28% [w/v] polyethylene glycol 3350