5Y00
Acid-tolerant monomeric GFP, Gamillus, fluorescence (ON) state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-16 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.900 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 161.158, 161.158, 161.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.960 - 1.600 |
| R-factor | 0.14595 |
| Rwork | 0.145 |
| R-free | 0.15831 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.039 |
| RMSD bond angle | 3.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.647 |
| Number of reflections | 90910 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium phosphate, sodium citrate, sodium chloride, HEPES, pH5.5 |
