5XZW

Crystal structure of Rad53 1-466

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	110
Detector technology	CCD
Collection date	2014-03-27
Detector	RAYONIX MX300HE
Wavelength(s)	0.90000
Spacegroup name	P 32 2 1
Unit cell lengths	115.809, 115.809, 141.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.528 - 2.800
R-factor	0.2686
Rwork	0.267
R-free	0.28430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.396
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.057	0.699
Number of reflections	27160
<I/σ(I)>	19.8
Completeness [%]	98.6	100
Redundancy	9	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	100MM CACODYLATE, 0.4M NACL, 1.5M (NH4)2SO4
1	VAPOR DIFFUSION	6.5	293	100MM CACODYLATE, 0.4M NACL, 1.5M (NH4)2SO4