5XZV

Crystal structure of Rad53 1-466 in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	110
Detector technology	CCD
Collection date	2016-07-20
Detector	RAYONIX MX300HE
Wavelength(s)	1.00000
Spacegroup name	P 32 2 1
Unit cell lengths	116.690, 116.690, 140.804
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.880 - 3.100
R-factor	0.273
Rwork	0.272
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.668
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.064	0.848
Number of reflections	20517
<I/σ(I)>	16.3
Completeness [%]	99.6	100
Redundancy	11	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	100MM CACODYLATE BUFFER, 0.4M NACL, 1.5M (NH4)2SO4