5XYF

Crystal structure of the human TIN2-TPP1-TRF2 telomeric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97854
Spacegroup name	C 2 2 21
Unit cell lengths	46.222, 112.258, 121.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.791 - 2.202
R-factor	0.197
Rwork	0.196
R-free	0.22680
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.124	0.080	0.571
Rmeas	0.135	0.088	0.622
Rpim	0.053	0.036	0.244
Total number of observations	106155
Number of reflections	16609
<I/σ(I)>	5.3
Completeness [%]	99.9	99.5	99.9
Redundancy	6.4	6	6.3
CC(1/2)		0.994	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1M Tris-HCl, pH 8.5, 0.2M Magnesium chloride, 25% PEG 3350, 2mM DTT