5XY9

Structure of the MST4 and 14-3-3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	72.994, 84.734, 111.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.821 - 2.303
R-factor	0.1985
Rwork	0.195
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qja
RMSD bond length	0.007
RMSD bond angle	0.916
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.097	0.878
Number of reflections	31310	1527
<I/σ(I)>	38	3
Completeness [%]	99.9	99.9
Redundancy	14	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2M MgCl2, 0.1M Tris pH 8.5, 25% PEG 3350