5XVV

Crystal Structure of Forward Inhibited Aspergillus niger Glutamate Dehydrogenase With Both Apo- and Alpha Ketoglutarate Bound Subunits

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-10-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	92.260, 92.150, 111.060
Unit cell angles	103.40, 93.95, 120.36

Refinement procedure

Resolution	33.000 - 2.250
R-factor	0.16029
Rwork	0.158
R-free	0.19863
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.433
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.350
High resolution limit [Å]	2.250	2.250
Rmerge	0.095	0.390
Number of reflections	135737	15991
<I/σ(I)>	7.96	2.1
Completeness [%]	95.5	92
Redundancy	2
CC(1/2)	0.990	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.15 M potassium bromide, 20% w/v PEG 2000 MME