5XV0
Crystal structure of Rib7 mutant D33N from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 117.098, 117.098, 380.382 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.24397 |
| Rwork | 0.242 |
| R-free | 0.28874 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xux |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.543 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.652 | |
| Number of reflections | 141352 | |
| <I/σ(I)> | 22.1 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10 % Tacsimate (pH 7.0), 10 % PEG 5000, and 100 mM HEPES (pH 7.0) |
