5XUP

Crystal structure of TRF1 and TERB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 64
Unit cell lengths	161.579, 161.579, 45.925
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.690 - 2.100
R-factor	0.179
Rwork	0.177
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bqo
RMSD bond length	0.006
RMSD bond angle	0.985
Data scaling software	HKL-2000
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.121	0.073	0.755
Rmeas	0.127	0.077	0.797
Rpim	0.040	0.025	0.254
Total number of observations	402507
Number of reflections	40395
<I/σ(I)>	3.1
Completeness [%]	100.0	100	100
Redundancy	10	9.6	9.8
CC(1/2)		0.995	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	10% 2-propanol, 0.1M Tris-HCl