5XSD

XylFII-LytSN complex mutant - D103A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-23
Detector	RAYONIX MX-225
Wavelength(s)	0.9798
Spacegroup name	P 41 21 2
Unit cell lengths	144.958, 144.958, 103.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.644 - 2.500
R-factor	0.2305
Rwork	0.229
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.028
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	38385
<I/σ(I)>	20.46
Completeness [%]	99.7
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Lithium sulfate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1000