5XQ2
Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 5A6 on the guide strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 21 3 |
Unit cell lengths | 201.843, 201.843, 201.843 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.950 - 3.330 |
R-factor | 0.254 |
Rwork | 0.252 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpy |
RMSD bond length | 0.013 |
RMSD bond angle | 1.703 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.380 |
High resolution limit [Å] | 3.320 | 3.320 |
Rmerge | 0.106 | 0.908 |
Number of reflections | 40665 | |
<I/σ(I)> | 45.5 | 4.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 33.8 | 26.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 308 | 2.8 M Na-acetate, pH7.0, 0.1M Tris-Cl, pH 8.0 and 0.15 M glycine |