5XPD
Sugar transporter of AtSWEET13 in inward-facing state with a substrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.780, 42.831, 116.553 |
| Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
| Resolution | 36.684 - 2.788 |
| R-factor | 0.2513 |
| Rwork | 0.247 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5CTG and 1FHH |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.962 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1819) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.788 |
| Rmerge | 0.148 |
| Number of reflections | 9248 |
| <I/σ(I)> | 5.8 |
| Completeness [%] | 96.3 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.9 | 293 | 100mM MES (pH 5.9), 38% (w/v) PEG 600, 100mM KH2PO4 |
