5XOX

Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-04
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97952
Spacegroup name	I 41
Unit cell lengths	164.703, 164.703, 275.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.841 - 3.000
R-factor	0.2403
Rwork	0.239
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3otb
RMSD bond length	0.014
RMSD bond angle	1.725
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.841	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.142	0.377
Number of reflections	68293	12656
<I/σ(I)>	17.87	3.02
Completeness [%]	93.4	84
Redundancy	5.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M MES pH 6.0, 0.9M Ammonium sulfate